Rabbit anti Human FIP Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/100-1/200 Protein Function Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
规格
产品名称
FIP Antibody
别名
上游刺激因子2抗体
英文别名
Class B basic helix-loop-helix protein 12 antibody | bHLHb12 antibody | FIP antibody | FOS interacting protein antibody | FOS-interacting protein antibody | Major late transcription factor 2 antibody | Upstream stimulatory factor 2 antibody | Upstream tra
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
FIP
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),牛(Cow),猪(Pig)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USF2.
阳性对照
WB: HeLa cell lysate. IHC: Human breast cancer tissue.
Hsp105/HSP110 Antibody (Ab108805) - Western Blot All lanes: Hsp105/HSP110 Antibody (Ab108805) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 96 kDa Observed band size: 103 kDa Exposure time: 61.0 s
Hsp105/HSP110 Antibody (Ab108805) - IF/ICC Immunofluorescent analysis of Hsp105/HSP110 Antibody (Ab108805) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Hsp105/HSP110 Antibody (Ab108805) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Hsp105/HSP110 Antibody (Ab108805) - IHC Immunohistochemical analysis of Hsp105/HSP110 Antibody (Ab108805) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.