Rabbit anti Human FKBP52 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-3 µg/mL
IHC: 5-30 μg/mL
IF/ICC: 1 μg/mL
Protein Function
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
WB: Jurkat, HEK293 and A375 cell lysates. IHC: Human stomach, prostate cancer, cerebrum, colorectal cancer, liver and lung cancer tissues. IF/ICC: HeLa cells.
FKBP52 Antibody (Ab103696) - Western Blot All lanes: FKBP52 Antibody (Ab103696) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 56, 53 kDa The expression profile and molecular weight observed may be due to phosphorylation. Exposure time: 25.3 s
FKBP52 Antibody (Ab103696) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: FKBP52 Antibody (Ab103696) at 30 µg/mL Antibody Second Ab: Caprine Anti-Rabbit IgG (HRP) at 2 µg/mL
FKBP52 Antibody (Ab103696) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: FKBP52 Antibody (Ab103696) at 30 µg/mL Antibody Second Ab: Caprine Anti-Rabbit IgG (HRP) at 2 µg/mL
FKBP52 Antibody (Ab103696) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: FKBP52 Antibody (Ab103696) at 30 µg/mL Antibody Second Ab: Caprine Anti-Rabbit IgG (HRP) at 2 µg/mL
FKBP52 Antibody (Ab103696) - IHC DAB staining on IHC Samples: Human Prostate cancer Tissue Primary Ab: FKBP52 Antibody (Ab103696) at 30 µg/mL Antibody Second Ab: Caprine Anti-Rabbit IgG (HRP) at 2 µg/mL
FKBP52 Antibody (Ab103696) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: FKBP52 Antibody (Ab103696) at 30 µg/mL Antibody Second Ab: Caprine Anti-Rabbit IgG (HRP) at 2 µg/mL
FKBP52 Antibody (Ab103696) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: FKBP52 Antibody (Ab103696) at 30 µg/mL Antibody Second Ab: Caprine Anti-Rabbit IgG (HRP) at 2 µg/mL
FKBP52 Antibody (Ab103696) - IF/ICC
IF analysis of FKBP52 Antibody (Ab103696) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with FKBP52 Antibody (Ab103696) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).