Rabbit anti Human FMRP Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
规格
产品名称
Recombinant FMRP Antibody
别名
脆性X综合征相关蛋白单克隆抗体 | 重组FMRP抗体
英文别名
FMR 1 antibody | Fmr1 antibody | Fmr1 gene antibody | FMR1_HUMAN antibody | FMRP antibody | Fragile X mental retardation 1 antibody | Fragile X mental retardation 1 protein antibody | Fragile X mental retardation protein 1 antibody | Fragile X mental reta
规格或纯度
ExactAb™, Validated, 重组, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
FMRP
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原
A synthetic peptide derived from human FMRP (AA 31-47)
阳性对照
WB: HEK293, U-2 OS, Jurkat, K562, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Recombinant FMRP Antibody (Ab103843) - Western Blot All lanes: Recombinant FMRP Antibody (Ab103843) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 71 kDa Observed band size: 64-80 kDa Exposure time: 22.8 s
Recombinant FMRP Antibody (Ab103843) - IF/ICC Immunocytochemistry analysis of FMRP (green) in HeLa using Recombinant FMRP Antibody (Ab103843), and cells were counterstained with DAPI (blue).
Recombinant FMRP Antibody (Ab103843) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant FMRP Antibody (Ab103843). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.