ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human Fragilis Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on.
Fragilis Antibody (Ab104100) - Western Blot
All lanes: Fragilis Antibody (Ab104100) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 100.0 s
Fragilis Antibody (Ab104100) - IF/ICC
Immunofluorescent analysis of IFITM3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Fragilis Antibody (Ab104100) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Fragilis Antibody (Ab104100) - IHC
Immunohistochemical analysis of IFITM3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Fragilis Antibody (Ab104100) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
1/500-1/1000
1/50 - 1/100
1/50 - 1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012960 |
分析证书 | Ab104100 |
| ZJ24F1012961 |
分析证书 | Ab104100 |
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