FSH-R Antibody (Ab104184) - Western Blot All lanes: FSH-R Antibody (Ab104184) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 78 kDa Observed band size: 72 kDa Exposure time: 26.5 s
FSH-R Antibody (Ab104184) - IHC Immunohistochemical analysis of FSHR staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FSH-R Antibody (Ab104184) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
FSH-R Antibody (Ab104184) - IF/ICC Immunofluorescent analysis of FSHR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FSH-R Antibody (Ab104184) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.