Fumarylacetoacetate hydrolase/FAA Antibody (Ab104242) - Western Blot All lanes: Fumarylacetoacetate hydrolase/FAA Antibody (Ab104242) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 46 kDa Observed band size: 41 kDa Exposure time: 17.4 s
Fumarylacetoacetate hydrolase/FAA Antibody (Ab104242) - IF/ICC Immunocytochemistry analysis of Fumarylacetoacetate hydrolase/FAA in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Fumarylacetoacetate hydrolase/FAA Antibody (Ab104242) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Fumarylacetoacetate hydrolase/FAA Antibody (Ab104242) - IHC Immunohistochemical analysis of Fumarylacetoacetate staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Fumarylacetoacetate hydrolase/FAA Antibody (Ab104242) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.