Rabbit anti Human FXR2 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on. Application: WB: 1/1500-1/1000 IHC:1/100 - 1/200 IF/ICC: 1/100-1/500 Protein Function: RNA-binding protein.
FXR2 Antibody (Ab104283) - Western Blot All lanes: FXR2 Antibody (Ab104283) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 90 kDa Exposure time: 52.5 s
FXR2 Antibody (Ab104283) - Western Blot All lanes: FXR2 Antibody (Ab104283) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 90 kDa Exposure time: 52.5 s
FXR2 Antibody (Ab104283) - IHC Immunohistochemical analysis of FXR2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FXR2 Antibody (Ab104283) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
FXR2 Antibody (Ab104283) - IF/ICC Immunofluorescent analysis of FXR2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FXR2 Antibody (Ab104283) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).