Rabbit anti Human Galectin 3 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 0.1-2 µg/mL IHC: 5-20 µg/mL ICC/IF: 5-20μg/mL Protein Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
Recombinant Galectin 3 expressed in E.coli (AA 1-250)
阳性对照
WB: C6, NIH/3T3, Hep G2, A549, HeLa, MCF7 and A431 cell lysates. IHC: Human colorectal cancer, lung cancer, ovarian cancer tissues; Porcine small intestine, kidney and spleen tissues. IHC: MCF7 cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified and Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
Galectin 3 Antibody (Ab104584) - Western Blot All lanes: Galectin 3 Antibody (Ab104584) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 29 kDa Exposure time: 0.1 s
Galectin 3 Antibody (Ab104584) - IF FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 μg/mL Galectin 3 Antibody (Ab104584) Second Ab: 1 μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Samples: Human Colorectal cancer Tissue Primary Ab: 10 μg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Samples: Human Ovarian cancer Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Galectin 3 Antibody (Ab104584) - IHC DAB staining on IHC Sample: Porcine Small intestine Tissue Primary Ab: 10 µg/mL Galectin 3 Antibody (Ab104584) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.