Recombinant GART Antibody (Ab104812) - Western Blot All lanes: Recombinant GART Antibody (Ab104812) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 107 kDa Observed band size: 116 kDa Exposure time: 172.2 s
Recombinant GART Antibody (Ab104812) - IHC Immunohistochemistry analysis of paraffin-embedded human cervix cancer using Recombinant GART Antibody (Ab104812). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant GART Antibody (Ab104812) - IF/ICC IF analysis of Recombinant GART Antibody (Ab104812) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GART Antibody (Ab104812) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).