Rabbit anti Human GATA3 Antibody, Recombinant, could be used for WB and IHC. Application: WB: 1/500 - 1/1000 IHC: 1/50 - 1/100 Protein Function Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Recombinant GATA3 Antibody (Ab104881) - Western Blot All lanes: Recombinant GATA3 Antibody (Ab104881) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 48&37 kDa Specific bands were detected for GATA3 full length at approximately 48kDa and the splice form at approximately 37kDa. Exposure time: 2.3 s
Recombinant GATA3 Antibody (Ab104881) - Western Blot All lanes: Recombinant GATA3 Antibody (Ab104881) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 48 kDa Observed band size: 48&37 kDa Specific bands were detected for GATA3 full length at approximately 48kDa and the splice form at approximately 37kDa. Exposure time: 4.9 s
Recombinant GATA3 Antibody (Ab104881) - IHC Immunohistochemistry analysis of paraffin-embedded Human bladder cancer using Recombinant GATA3 Antibody (Ab104881)(1/50). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.