Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
宿主种属
兔(Rabbit)
特异性
GATA4
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属
狗(Dog)
免疫原
A synthetic peptide derived from human GATA4 (AA 5-48)
阳性对照
WB: Hep G2, HEK293 and HeLa cell lysates. IF/ICC: Hep G2 cells.
Recombinant GATA4 Antibody (Ab104893) - Western Blot All lanes: Recombinant GATA4 Antibody (Ab104893) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution Predicted band size: 45 kDa Observed band size: 22, 57 kDa ~20 kDa band is consistent with what has been described in the literature (PMID: 25501827). Caspase-1 mediated cleavage of GATA4 generates a truncated protein that retains the ability to bind DNA but lacks transcriptional activation domains and acts as a dominant negative regulator of GATA4. Exposure time: 167.7 s
Recombinant GATA4 Antibody (Ab104893) - IF/ICC Immunofluorescent analysis of Hep G2 cells, using Recombinant GATA4 Antibody (Ab104893).
ZJ25F0318762
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