Rabbit anti Human Ghrelin Antibody, Polyclonal, could be used for WB, IF, ICC, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 ICC/IF: 1/100-1/300 Protein Function: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
A synthetic peptide derived from human Ghrelin (AA 47-96)
阳性对照
WB: HEK293, THP-1, Hep G2 and LNCaP cell lysates. IF/ICC: A549 cells. IHC: Human lung cancer tissue.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Immunogen affinity purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Lyophilized:Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. It is recommended that a thawed sample is stored at 4°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by s
Ghrelin Antibody (Ab105341) - Western Blot All lanes: Ghrelin Antibody (Ab105341) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time: 0.3 s
Ghrelin Antibody (Ab105341) - IHC Immunohistochemical analysis of paraffin-embedded Human lung cancer. Ghrelin Antibody (Ab105341) was diluted at 1/100 (4° overnight). High-pressure and temperature Tris-EDTA, pH 8.0 was used for antigen retrieval. Negative control (right) obtained from antibody was pre-absorbed by immunogen peptide.
Ghrelin Antibody (Ab105341) - IHC Immunohistochemistry analysis of Ghrelin Antibody (Ab105341) in paraffin-embedded human lung carcinoma tissue.
Ghrelin Antibody (Ab105341) - IF/ICC Immunofluorescence analysis of A549 cells. Ghrelin Antibody (Ab105341) was diluted at 1/200 (4°C overnight). Goat Anti Rabbit IgG (H&L) - AF488 Secondary antibody was diluted at 1/1000 (room temperature, 50min).