Rabbit anti Human GIT1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
规格
产品名称
GIT1 Antibody
别名
GIT1抗体 | GIT1抗体
英文别名
ARF GAP GIT1 | ARF GTPase-activating protein GIT1 | CAT1 | CAT-1 | Cool-associated and tyrosine-phosphorylated protein 1 | G protein-coupled receptor kinase interacting ArfGAP 1 | G protein-coupled receptor kinase interactor 1 | G protein-coupled receptor
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
GIT1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human GIT1
阳性对照
WB: A549, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
GIT1 Antibody (Ab105406) - Western Blot All lanes: GIT1 Antibody (Ab105406) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 90 kDa Exposure time: 60.0 s
GIT1 Antibody (Ab105406) - Western Blot All lanes: GIT1 Antibody (Ab105406) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 84 kDa Observed band size: 86 kDa Exposure time: 60.0 s
GIT1 Antibody (Ab105406) - IHC Immunohistochemical analysis of GIT1 Antibody (Ab105406) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the GIT1 Antibody (Ab105406) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.