Rabbit anti Human GNB1 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
规格
产品名称
GNB1 Antibody
别名
G蛋白/鸟苷酸结合蛋白抗体 | GNB1抗体 | GNB1多抗 | GNB1抗体
英文别名
G protein beta subunit GI/GS/GT | guanine nucleotide binding protein (G protein), beta polypeptide 1 | guanine nucleotide-binding protein G | GBB1_HUMAN | Beta subunit signal transducing proteins GS/GI antibody | G protein beta 1 subunit antibody | GBB1 a
规格或纯度
ExactAb™, Validated, Carrier Free, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
GNB1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Fusion protein of human GNB1 (AA 100-340).
阳性对照
WB: Hep G2, A549, HEK293, Jurkat, HeLa, Raji, NIH/3T3 and C6 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells
GNB1 Antibody (Ab105938) - Western Blot All lanes: GNB1 Antibody (Ab105938) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 60.0 s
GNB1 Antibody (Ab105938) - IHC Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GNB1 Antibody (Ab105938). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
GNB1 Antibody (Ab105938) - IF/ICC
IF analysis of GNB1 Antibody (Ab105938) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with GNB1 Antibody (Ab105938) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ23F1202121
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