Rabbit anti Human GNB2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.
Recombinant GNB2 Antibody (Ab105941) - Western Blot All lanes: Recombinant GNB2 Antibody (Ab105941) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa Observed band size: 32 kDa Exposure time: 50.7 s
Recombinant GNB2 Antibody (Ab105941) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant GNB2 Antibody (Ab105941). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant GNB2 Antibody (Ab105941) - IF/ICC
IF analysis of Recombinant GNB2 Antibody (Ab105941) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GNB2 Antibody (Ab105941) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ23F1202151
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