This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
宿主种属
兔(Rabbit)
特异性
GPI-PLD
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPIPLD.
阳性对照
WB: Mouse brian lysates. IHC: Human kidney cancer tissue. IF/ICC: MCF7 cells.
GPI-PLD Antibody (Ab106204) - Western Blot All lanes: GPI-PLD Antibody (Ab106204) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 92 kDa Observed band size: 121 kDa Exposure time: 20.0 s
GPI-PLD Antibody (Ab106204) - IF/ICC Immunofluorescent analysis of GPIPLD staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GPI-PLD Antibody (Ab106204) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
GPI-PLD Antibody (Ab106204) - IHC Immunohistochemical analysis of GPIPLD staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GPI-PLD Antibody (Ab106204) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.