ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human GPR 174 Antibody, Polyclonal, could be used for WB, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IF/ICC: 1/100-1/500
Protein Function
Putative receptor for purines coupled to G-proteins.
GPR 174 Antibody (Ab106230) - Western Blot
All lanes: GPR 174 Antibody (Ab106230) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 41 kDa
Exposure time: 100.0 s
GPR 174 Antibody (Ab106230) - IF/ICC
Immunofluorescent analysis of GPR 174 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GPR 174 Antibody (Ab106230) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with AF647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
1/100-1/500
1/500-1/1000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910097 |
分析证书 | Ab106230 |
| ZJ24F0910098 |
分析证书 | Ab106230 |
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