Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
宿主种属
兔(Rabbit)
特异性
GSK3 beta
种属反应性
人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human GSK3 beta (AA 1-13)
阳性对照
WB: Jurkat, HeLa, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human cholangiocarcinoma. IF/ICC: HeLa cells.
Recombinant GSK3 beta Antibody (Ab106628) - Western Blot All lanes: Recombinant GSK3 beta Antibody (Ab106628) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 10.7 s
Recombinant GSK3 beta Antibody (Ab106628) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant GSK3 beta Antibody (Ab106628). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF/ICC: Recombinant GSK3 beta Antibody (Ab106628)
IF analysis of Recombinant GSK3 beta Antibody (Ab106628) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GSK3 beta Antibody (Ab106628) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F0202832
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