ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human GTF2E2 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
GTF2E2 Antibody (Ab106705) - Western Blot
All lanes: GTF2E2 Antibody (Ab106705) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 26, 31, 32 kDa
Exposure time: 91.2 s
GTF2E2 Antibody (Ab106705) - IHC
Immunohistochemical analysis of GTF2E2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with GTF2E2 Antibody (Ab106705) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
GTF2E2 Antibody (Ab106705) - IF/ICC
Immunofluorescent analysis of GTF2E2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with GTF2E2 Antibody (Ab106705) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
1/50-1/200
1/500-1/1000
1/50-1/100
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012916 |
分析证书 | Ab106705 |
| ZJ24F1012917 |
分析证书 | Ab106705 |
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