ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human HADH Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
HADH Antibody (Ab106795) - Western Blot
All lanes: HADH Antibody (Ab106795) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 30, 34 kDa
The multiple bands are isoforms produced by alternative splicing. HADH Antibody (Ab106795) recognizes different isoforms of HADH.
Exposure time: 44.1 s
HADH Antibody (Ab106795) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using HADH Antibody (Ab106795). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HADH Antibody (Ab106795) - IF/ICC
IF analysis of HADH Antibody (Ab106795) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with HADH Antibody (Ab106795) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/500-1/1000
1/50-1/100
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0318731 |
分析证书 | Ab106795 |
| ZJ25F0318730 |
分析证书 | Ab106795 |
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