Recombinant Hexokinase II Antibody (Ab107380) - Western Blot All lanes: Recombinant Hexokinase II Antibody (Ab107380) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution Predicted band size: 102 kDa Observed band size: 99 kDa Exposure time: 6.2 s
Recombinant Hexokinase II Antibody (Ab107380) - IHC Immunohistochemical analysis of Hexokinase II staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant Hexokinase II Antibody (Ab107380) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.