Recombinant HIF1 beta Antibody (Ab107495) - Western Blot All lanes: Recombinant HIF1 beta Antibody (Ab107495) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa Observed band size: 91 kDa Exposure time: 37.2 s
Recombinant HIF1 beta Antibody (Ab107495) - Western Blot All lanes: Recombinant HIF1 beta Antibody (Ab107495) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 87 kDa Observed band size: 91 kDa Exposure time: 37.2 s
Recombinant HIF1 beta Antibody (Ab107495) - IHC Immunohistochemical analysis of HIF1 beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HIF1 beta Antibody (Ab107495) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.