Basic helix loop helix PAS protein MOP2 antibody | Basic-helix-loop-helix-PAS protein MOP2 antibody | bHLHe73 antibody | Class E basic helix-loop-helix protein 73 antibody | ECYT4 antibody | Endothelial PAS domain containing protein 1 antibody | Endotheli
规格或纯度
已验证, ExactAb™, 1.0 mg/mL
生化机理
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
宿主种属
兔(Rabbit)
特异性
HIF-2-alpha
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human EPAS-1 around the non-acetylation site of K385.
阳性对照
WB: HEK293, MCF7 and SH-SY5Y cell lysates. IHC: Human mammary cancer and colon tissues. ICC/IF: Human stomach and Mouse spleen tissues.
HIF-2-alpha Antibody (Ab107509) - Western Blot All lanes: HIF-2-alpha Antibody (Ab107509) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 96 kDa Observed band size: 100 kDa Exposure time: 28.3 s
HIF-2-alpha Antibody (Ab107509) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HIF-2-alpha Antibody (Ab107509) was diluted at 1/100. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HIF-2-alpha Antibody (Ab107509) - IHC Immunohistochemistry analysis of paraffin-embedded Human mammary-cancer tissue using HIF-2-alpha Antibody (Ab107509) was diluted at 1/100. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of human-stomach tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of human-stomach tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of mouse-spleen tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
HIF-2-alpha Antibody (Ab107509) - IF/ICC Immunofluorescence analysis of mouse-spleen tissue. HIF-2-alpha Antibody (Ab107509) (red) was diluted at 1/200 (4°C,overnight). Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).