Recombinant Histone H2A.X Antibody

Name: Recombinant Histone H2A.X Antibody
SKU: Ab107657
Availability: InStock

货号: Ab107657

COA

有货

级别和纯度: 重组 ? ExactAb™ ? 已验证 ? 见COA

应用

IF/ICC, IHC, WB

宿主种属

兔(Rabbit)

种属反应性

人(Human), 大鼠(Rat), 小鼠(Mouse)

亚型

Rabbit IgG

偶联

Unconjugated

规格

库存

价格

数量

10μl

Ab107657-10μl

现货

¥374.90

50μL

Ab107657-50μl

现货

¥974.90

100μL

Ab107657-100μl

现货

¥1,574.90

1ml

Ab107657-1ml

期货

¥16,999.90

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概述规格储存与运输图片应用技术文档高级数据关联靶点（人）质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器常见问题

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为什么选择此级别

ExactAb™,已验证,重组级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Rabbit anti Human Histone H2A.X Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

规格

产品名称

Recombinant Histone H2A.X Antibody

别名

英文别名

规格或纯度

重组, ExactAb™, 已验证, 见COA

生化机理

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

宿主种属

兔(Rabbit)

特异性

Histone H2A.X

种属反应性

人(Human),大鼠(Rat),小鼠(Mouse)

免疫原

A synthetic peptide derived from human Histone H2A.X (AA 12-48)

阳性对照

WB: Mouse kidney, Rat brain, Raji, Ramos, HeLa and HEK293 lysates. IHC: Rat stomach, pancreas, lung, Human Hodgkin's lymphoma, colon cancer, Mouse spleen tissues. IF/ICC: HeLa cells.

偶联

Unconjugated

克隆类型

重组抗体

Format

Whole IgG

亚型

Rabbit IgG

轻链亚型

kappa

纯化方法

Protein A purified

浓度

见COA

分子类型

抗体

产品线

生物产品

储存与运输

物理外观

Liquid

储存缓冲液

10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4

防腐剂

0.02% Sodium azide

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。

图片

Western Blot: Recombinant Histone H2A.X Antibody (Ab107657)

Recombinant Histone H2A.X Antibody (Ab107657) - Western Blot
All lanes: Recombinant Histone H2A.X Antibody (Ab107657) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression.The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.
Exposure time: 11.3 s

IF/ICC: Recombinant Histone H2A.X Antibody (Ab107657)

Recombinant Histone H2A.X Antibody (Ab107657) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Histone H2A.X Antibody (Ab107657).