Rabbit anti Human HMGB2 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on.
规格
产品名称
HMGB2 Antibody
别名
高迁移率族蛋白2抗体 | Anti-HMGB2抗体 | HMGB2抗体
英文别名
C80539 antibody | High mobility group (nonhistone chromosomal) protein 2 antibody | High mobility group box 2 antibody | High mobility group protein 2 antibody | High mobility group protein B2 antibody | HMG 2 antibody | HMG B2 antibody | HMG-2 antibody |
规格或纯度
ExactAb™, 已验证, 无载体, 1.0 mg/mL
生化机理
与染色质结合的 DNA 结合蛋白,具有弯曲 DNA 的能力。优先结合单链 DNA。作为 RAG 复合物的辅助因子参与 V(D)J 重组。在保守重组信号序列(RSS)的 23 bp 间距处通过刺激裂解和 RAG 蛋白结合发挥作用。
宿主种属
兔(Rabbit)
特异性
HMGB2
种属反应性
人(Human),小鼠(Mouse),牛(Cow)
免疫原
A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HMGB2.
阳性对照
WB: HEL and Jurkat cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells
HMGB2 Antibody (Ab108314) - Western Blot All lanes: HMGB2 Antibody (Ab108314) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/30000 dilution
Predicted band size: 24 kDa Observed band size: 27 kDa Exposure time: 60.0 s
HMGB2 Antibody (Ab108314) - IHC Immunohistochemical analysis of HMGB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with HMGB2 Antibody (Ab108314) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
HMGB2 Antibody (Ab108314) - IF/ICC Immunofluorescent analysis of HMGB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with HMGB2 Antibody (Ab108314) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.