DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
宿主种属
兔(Rabbit)
特异性
HSF1
种属反应性
人(Human)
免疫原
A synthetic peptide of human HSF1
阳性对照
WB: HeLa, Jurkat, Hep G2 and MCF7 cell lysates. IHC: Human breast carcinoma tissue. IF/ICC: HeLa cells.
Recombinant HSF1 Antibody (Ab108785) - Western Blot All lanes: Recombinant HSF1 Antibody (Ab108785) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution Predicted band size: 57 kDa Observed band size: 72 kDa Exposure time: 284.5 s
Recombinant HSF1 Antibody (Ab108785) - IHC Immunohistochemical analysis of HSF1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HSF1 Antibody (Ab108785) at room temperature and detected using an HRP conjugated compact polymer system.
Recombinant HSF1 Antibody (Ab108785) - IF/ICC IF analysis of Recombinant HSF1 Antibody (Ab108785) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HSF1 Antibody (Ab108785) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).