In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.Tissue specificity: HSPA1B is testis-specific.
宿主种属
兔(Rabbit)
特异性
HSPA1A, HSPA1B
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Hsp70 (AA 400-641)
表位
Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 437-479 of human Hsp 70.
阳性对照
WB: Mouse heart, Mouse liver, HeLa, K562, A549, NIH/3T3 and C6 lysates. IHC: Human squamous carcinoma, testis cancer, prostate cancer, breast tissues. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Hsp70 Antibody (Ab108909) - Western Blot All lanes: Recombinant Hsp70 Antibody (Ab108909) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 71 kDa Exposure time: 7.7 s
Recombinant Hsp70 Antibody (Ab108909) - Western Blot All lanes: Recombinant Hsp70 Antibody (Ab108909) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 71 kDa Exposure time: 7.5 s
Recombinant Hsp70 Antibody (Ab108909) - Western Blot All lanes: Recombinant Hsp70 Antibody (Ab108909) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 71 kDa Exposure time: 4.4 s
Recombinant Hsp70 Antibody (Ab108909) - IHC Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using Recombinant Hsp70 Antibody (Ab108909) at 1/200 dilution.
Recombinant Hsp70 Antibody (Ab108909) - IHC Immunohistochemical analysis of paraffin-embedded Human testis cancer, using Recombinant Hsp70 Antibody (Ab108909) at 1/200 dilution.
Recombinant Hsp70 Antibody (Ab108909) - IHC Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using Recombinant Hsp70 Antibody (Ab108909) at 1/200 dilution.
Recombinant Hsp70 Antibody (Ab108909) - IHC Immunohistochemical analysis of paraffin-embedded human breast, using Recombinant Hsp70 Antibody (Ab108909).
Recombinant Hsp70 Antibody (Ab108909) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Hsp70 Antibody (Ab108909).
ZJ25F0319019
危险品化学品经营许可证（带存储）