Recombinant HSPA12A Antibody (Ab108950) - Western Blot All lanes: Recombinant HSPA12A Antibody (Ab108950) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 100.0 s
Recombinant HSPA12A Antibody (Ab108950) - Western Blot All lanes: Recombinant HSPA12A Antibody (Ab108950) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa Exposure time: 34.7 s
Recombinant HSPA12A Antibody (Ab108950) - IHC Immunohistochemical analysis of paraffin-embedded mouse brain, using Recombinant HSPA12A Antibody (Ab108950).
Recombinant HSPA12A Antibody (Ab108950) - IF/ICC IF analysis of Recombinant HSPA12A Antibody (Ab108950) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HSPA12A Antibody (Ab108950) at 1/50 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).