Rabbit anti Human HSPA2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
规格
产品名称
Recombinant HSPA2 Antibody
别名
热休克蛋白70蛋白2抗体 | 重组HSPA2抗体
英文别名
Heat shock 70 kDa protein 2 | Heat shock 70 kDa protein 2 | heat shock 70kD protein 2 | heat shock 70kDa protein 2 | heat shock-related 70 kDa protein 2 | HSP70-2 | HSP70-3 | HSPA2 | 70kDa antibody | Hcp70.2 antibody | Heat shock 70 kDa protein 2 antibody
规格或纯度
ExactAb™, Validated, 重组, 0.8 mg/mL
宿主种属
兔(Rabbit)
特异性
HSPA2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human HSPA2 (AA 488-637)
阳性对照
WB: A549, A431, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.
Recombinant HSPA2 Antibody (Ab108960) - Western Blot All lanes: Recombinant HSPA2 Antibody (Ab108960) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 70 kDa Observed band size: 71 kDa Exposure time: 29.1 s
Recombinant HSPA2 Antibody (Ab108960) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant HSPA2 Antibody (Ab108960). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant HSPA2 Antibody (Ab108960) - IF/ICC
IF analysis of Recombinant HSPA2 Antibody (Ab108960) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HSPA2 Antibody (Ab108960) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
ZJ24F0506061
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