Rabbit anti Human Ihh Antibody, Recombinant, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
规格
产品名称
Recombinant Ihh Antibody
别名
Ihh重组兔单克隆抗体 | 重组Ihh抗体
英文别名
BDA1 antibody | HHG-2 antibody | HHG2 antibody | IHH antibody | IHH_HUMAN antibody | Indian Hedgehog antibody | Indian hedgehog homolog antibody | Indian hedgehog protein antibody | Indian hedgehog protein C-product antibody | Indian hedgehog protein N-pr
规格或纯度
ExactAb™, Validated, 重组, 0.3 mg/mL
宿主种属
兔(Rabbit)
特异性
Ihh
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Ihh (AA 28-42)
阳性对照
WB: Hep G2, LNCaP, Jurkat, HEK293, NIH/3T3 and C6 cell lysates. IHC: Mouse colon tissue. IF/ICC: HeLa cells.
Recombinant Ihh Antibody (Ab109665) - Western Blot All lanes: Recombinant Ihh Antibody (Ab109665) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 41 kDa Exposure time: 10.7 s
Recombinant Ihh Antibody (Ab109665) - IHC Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant Ihh Antibody (Ab109665). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Ihh Antibody (Ab109665) - IF/ICC
IF analysis of Recombinant Ihh Antibody (Ab109665) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Ihh Antibody (Ab109665) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ23F1202163
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