Rabbit anti Human IL-1RA Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Recombinant IL-1RA Antibody (Ab110059) - Western Blot All lanes: Recombinant IL-1RA Antibody (Ab110059) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 18, 23 kDa Exposure time: 0.9 s
Recombinant IL-1RA Antibody (Ab110059) - IF/ICC Immunocytochemistry analysis of IL 1 Receptor Antagonist Protein (green) in Hep G2 using Recombinant IL-1RA Antibody (Ab110059), and cells were counterstained with DAPI (blue).
Recombinant IL-1RA Antibody (Ab110059) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant IL-1RA Antibody (Ab110059). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.