Rabbit anti Human INSL4 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/200
ICC/IF: 1/100-1/500
Protein Function
May play an important role in trophoblast development and in the regulation of bone formation.
规格
产品名称
INSL4 Antibody
别名
早期胎盘胰岛素样肽INSL4抗体 | INSL4 抗体
英文别名
early placenta insulin like peptide (EPIL) antibody | Early placenta insulin like peptide A chain antibody | Early placenta insulin like peptide antibody | Early placenta insulin like peptide B chain antibody | Early placenta insulin-like peptide A chain
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
INSL4
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human INSL4.
阳性对照
WB: HT-29 and COLO 205 cell lysates. IHC: Human placenta tissue. IF/ICC: A549 cells.
INSL4 Antibody (Ab110689) - Western Blot All lanes: INSL4 Antibody (Ab110689) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 15 kDa Observed band size: 17 kDa Exposure time: 91.6 s
INSL4 Antibody (Ab110689) - IHC Immunohistochemical analysis of INSL4 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with INSL4 Antibody (Ab110689) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
INSL4 Antibody (Ab110689) - IF/ICC Immunofluorescent analysis of INSL4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with INSL4 Antibody (Ab110689) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.