Rabbit anti Human Insulin degrading enzyme/IDE Antibody, Recombinant, could be used for WB, IHC and so on. Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716) - Western Blot All lanes: Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 118 kDa Observed band size: 106 kDa Exposure time: 2.9 s
Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716) - IF/ICC IF analysis of Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Insulin degrading enzyme/IDE Antibody (Ab110716) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).