Rabbit anti Human Integrin alpha 6 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200 Protein Function Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
Recombinant Integrin alpha 6 Antibody (Ab110811) - Western Blot All lanes: Recombinant Integrin alpha 6 Antibody (Ab110811) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 125 kDa Observed band size: 124 kDa Exposure time: 16.1 s
Recombinant Integrin alpha 6 Antibody (Ab110811) - IHC Immunohistochemistry analysis of paraffin-embedded Human uterus using Recombinant Integrin alpha 6 Antibody (Ab110811). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF analysis of Recombinant Integrin alpha 6 Antibody (Ab110811) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Integrin alpha 6 Antibody (Ab110811) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
ZJ24F0911344
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