Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
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Involucrin Antibody (Ab111039) - Western Blot All lanes: Involucrin Antibody (Ab111039) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 68 kDa Observed band size: 110, 29 kDa The predicted MW of involucrin is 68 kDa, but it also forms 120 kDa dimers, while different reports provided variable results ranging from 90 - 140 kDa. (PMID: 11099111, 1503502, 12150517).Research also has shown that the C-terminal fragment of involucrin (~30 kDa) can participate in repair through cross-linking in a model of epidermal barrier damage (PMID: 9208334). Exposure time: 120.0 s
Involucrin Antibody (Ab111039) - IHC Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Involucrin Antibody (Ab111039). The picture on the right is blocked with the synthesized peptide.