Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Post-translational: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
宿主种属
兔(Rabbit)
特异性
Lamin B1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Lamin B1 (AA 500-550).
阳性对照
WB: Mouse brain, Rat heart, Hep G2, Ramos, HUVEC, SH-SY5Y and MOLT-4 lysates. IHC: Rat ovary, liver, Human non-Hodgkin's lymphoma, glioblastoma, prostate cancer tissue. IF/ICC: HeLa, Hep G2, Human colon cancer cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Lamin B1 Antibody (Ab112660) - Western Blot All lanes: Recombinant Lamin B1 Antibody (Ab112660) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 64 kDa Exposure time: 1.76 s
Recombinant Lamin B1 Antibody (Ab112660) - Western Blot All lanes: Recombinant Lamin B1 Antibody (Ab112660) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 64 kDa Exposure time: 33.4 s
Recombinant Lamin B1 Antibody (Ab112660) - IHC Immunohistochemical analysis of paraffin-embedded Rat ovary, using Recombinant Lamin B1 Antibody (Ab112660) at 1/200 dilution.
Recombinant Lamin B1 Antibody (Ab112660) - IHC Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using Recombinant Lamin B1 Antibody (Ab112660) at 1/100 dilution.
Recombinant Lamin B1 Antibody (Ab112660) - IHC Immunohistochemical analysis of paraffin-embedded Human glioblastoma, using Recombinant Lamin B1 Antibody (Ab112660) at 1/100 dilution.
Recombinant Lamin B1 Antibody (Ab112660) - IHC Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using Recombinant Lamin B1 Antibody (Ab112660) at 1/100 dilution.
Recombinant Lamin B1 Antibody (Ab112660) - IHC Immunohistochemical analysis of paraffin-embedded rat liver, using Recombinant Lamin B1 Antibody (Ab112660) .
Recombinant Lamin B1 Antibody (Ab112660) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Lamin B1 Antibody (Ab112660) at 1/150 dilution.
Recombinant Lamin B1 Antibody (Ab112660) - IF/ICC Immunofluorescent analysis of Hep G2 cells, using Recombinant Lamin B1 Antibody (Ab112660).
Recombinant Lamin B1 Antibody (Ab112660) - IF/ICC Immunohistofluorescence analysis of Human colon cancer, using Recombinant Lamin B1 Antibody (Ab112660) at 1/100 dilution.