ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human LEF1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
ICC/IF: 1/50 - 1/200
Protein Function
Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Recombinant LEF1 Antibody (Ab112963) - Western Blot
All lanes: Recombinant LEF1 Antibody (Ab112963) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 25-58 kDa
Exposure time: 150.0 s
Recombinant LEF1 Antibody (Ab112963) - Western Blot
All lanes: Recombinant LEF1 Antibody (Ab112963) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 25-58 kDa
Exposure time: 150.0 s
Recombinant LEF1 Antibody (Ab112963) - IF/ICC
Immunocytochemistry analysis of LEF1 (green) in Jurkat cells using Recombinant LEF1 Antibody (Ab112963), and cells were counterstained with DAPI (blue).
1/500-1/1000
1/50-1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1011847 |
分析证书 | Ab112963 |
| ZJ23F1001429 |
分析证书 | Ab112963 |
| ZJ23F1001430 |
分析证书 | Ab112963 |
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查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 →
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