ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human MAGEA1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/100 - 1/200
IF/ICC: 1/200
Protein Function
May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
MAGEA1 Antibody (Ab114011) - Western Blot
All lanes: MAGEA1 Antibody (Ab114011) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 44 kDa
Exposure time: 0.3 s
MAGEA1 Antibody (Ab114011) - IHC
Immunohistochemical analysis of MAGEA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MAGEA1 Antibody (Ab114011) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
MAGEA1 Antibody (Ab114011) - IF/ICC
IF analysis of MAGEA1 Antibody (Ab114011) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MAGEA1 Antibody (Ab114011) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/100 - 1/200
1/200
1/500-1/1000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012520 |
分析证书 | Ab114011 |
| ZJ24F1012521 |
分析证书 | Ab114011 |
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