ATG8E antibody | Autophagy related protein LC3 A antibody | Autophagy related ubiquitin like modifier LC3 A antibody | Autophagy-related protein LC3 A antibody | Autophagy-related ubiquitin-like modifier LC3 A antibody | LC3 antibody | LC3A antibody | MAP
规格或纯度
ExactAb™, Validated, 重组, 0.3 mg/mL
宿主种属
兔(Rabbit)
特异性
MAP1LC3A
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human MAP1LC3A (AA 101-120).
阳性对照
WB: NIH/3T3, HeLa, C6, Mouse heart, Mouse brain lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Recombinant MAP1LC3A Antibody (Ab114168) - Western Blot All lanes: Recombinant MAP1LC3A Antibody (Ab114168) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa Observed band size: 14, 16 kDa Exposure time: 57.2 s
Recombinant MAP1LC3A Antibody (Ab114168) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MAP1LC3A Antibody (Ab114168). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant MAP1LC3A Antibody (Ab114168) - IF/ICC
IF analysis of Recombinant MAP1LC3A Antibody (Ab114168) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MAP1LC3A Antibody (Ab114168) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ26F0737172
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