Rabbit anti Human MAPK6/ERK3 Antibody, Recombinant, could be used for WB, IHC and so on.
Application: WB: 1/500-1/2000 IHC: 1/50-1/100 Protein Function: Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
Recombinant MAPK6/ERK3 Antibody (Ab114239) - Western Blot All lanes: Recombinant MAPK6/ERK3 Antibody (Ab114239) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 83 kDa Observed band size: 88 kDa Exposure time: 99.0 s
Recombinant MAPK6/ERK3 Antibody (Ab114239) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant MAPK6/ERK3 Antibody (Ab114239). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IF analysis of Recombinant MAPK6/ERK3 Antibody (Ab114239) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MAPK6/ERK3 Antibody (Ab114239) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).