Recombinant MDH2 Antibody (Ab114702) - Western Blot All lanes: Recombinant MDH2 Antibody (Ab114702) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa Observed band size: 33 kDa Exposure time: 13.9 s
Recombinant MDH2 Antibody (Ab114702) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MDH2 Antibody (Ab114702). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant MDH2 Antibody (Ab114702) - IF/ICC
IF analysis of Recombinant MDH2 Antibody (Ab114702) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MDH2 Antibody (Ab114702) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).