ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human MEK1/MEK2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/5000-1/10000
IHC: 1/100-1/500
ICC/IF: 1/50-1/100
Protein Function：
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Recombinant MEK1/MEK2 Antibody (Ab114851) - Western Blot
All lanes: Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 42 kDa
Exposure time: 1.1 s
Recombinant MEK1/MEK2 Antibody (Ab114851) - Western Blot
All lanes use Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/5000 dilution for 1 hour at room temperature.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/50 dilution.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/150 dilution.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851) at 1/200 dilution.
Recombinant MEK1/MEK2 Antibody (Ab114851) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK1/MEK2 Antibody (Ab114851).
Recombinant MEK1/MEK2 Antibody (Ab114851) - IHC
Immunohistochemical analysis of paraffin-embedded human kidney, using Recombinant MEK1/MEK2 Antibody (Ab114851).
1/5000 – 1/10000
1/100-1/500
1/50-1/100
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910849 |
分析证书 | Ab114851 |
| ZJ25F0319565 |
分析证书 | Ab114851 |
| ZJ24F0910850 |
分析证书 | Ab114851 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 High Performance 级别指南 →
危险品化学品经营许可证（带存储）