ExactAb™,已验证,敲低验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
| Rabbit anti Human MEK3/MEK6 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Application: |
| WB: 1/1000-1/2000 IHC: 1/100-1/200 |
| Protein Function: |
| Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
Recombinant MEK3/MEK6 Antibody (Ab114875) - Western Blot
All lanes: Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 36, 38 kDa
Recombinant MEK3/MEK6 Antibody (Ab114875) recognizes two isoforms of MEK3/MEK6.
Exposure time: 30.0 s
Recombinant MEK3/MEK6 Antibody (Ab114875) - Western Blot
All lanes: Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 36, 38 kDa
Recombinant MEK3/MEK6 Antibody (Ab114875) recognizes two isoforms of MEK3/MEK6.
Exposure time: 30.0 s
Recombinant MEK3/MEK6 Antibody (Ab114875) - Western Blot
All lanes: Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa
Exposure time: 30.0 s
Recombinant MEK3/MEK6 Antibody (Ab114875) - IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen, using Recombinant MEK3/MEK6 Antibody (Ab114875).
Recombinant MEK3/MEK6 Antibody (Ab114875) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant MEK3/MEK6 Antibody (Ab114875).
Recombinant MEK3/MEK6 Antibody (Ab114875) - IF/ICC
IF analysis of Recombinant MEK3/MEK6 Antibody (Ab114875) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MEK3/MEK6 Antibody (Ab114875) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/100 - 1/200
1/1000 - 1/2000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012705 |
分析证书 | Ab114875 |
| ZJ24F1012706 |
分析证书 | Ab114875 |
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查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 KD Validation 级别指南 →
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