ExactAb™,已验证 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human MEK5 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/1000-1/2000
IHC: 1/200-1/500
Protein Function：
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
MEK5 Antibody (Ab114893) - Western Blot
All lanes: MEK5 Antibody (Ab114893) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 49 kDa
Exposure time: 100.0 s
MEK5 Antibody (Ab114893) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Breast Carcinoma tissue using MEK5 Antibody (Ab114893). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
MEK5 Antibody (Ab114893) - IF/ICC
IF analysis of MEK5 Antibody (Ab114893) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MEK5 Antibody (Ab114893) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
1/1000-1/2000
1/200-1/500
1/300
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1113887 |
分析证书 | Ab114893 |
| ZJ24F1113888 |
分析证书 | Ab114893 |
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