Rabbit anti Human Methionine Aminopeptidase 2/p67 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/1000-1/2000 Protein Function: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
Recombinant Methionine Aminopeptidase 2/p67 Antibody (Ab115120) - Western Blot All lanes: Recombinant Methionine Aminopeptidase 2/p67 Antibody (Ab115120) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 53 kDa Observed band size: 61 kDa Exposure time: 12.8 s