Rabbit anti Human MGMT Antibody, Polyclonal antibody, could be used for WB, IHC and so on. Application WB: 0.5-3µg/mL IHC: 5-30µg/mL Protein Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
规格
产品名称
MGMT Antibody
别名
兔抗人MGMT抗体 | O6甲基鸟嘌呤DNA甲基转移酶抗体 | MGMT抗体
英文别名
6 O methylguanine DNA methyltransferase antibody | 6-O-methylguanine-DNA methyltransferase antibody | Agat antibody | AGT antibody | AI267024 antibody | EC 2.1.1.63 antibody | Methylated DNA protein cysteine methyltransferase antibody | Methylated-DNA--pr
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属
兔(Rabbit)
特异性
MGMT
种属反应性
人(Human)
免疫原
Recombinant MGMT expressed in E.coli (AA 1-207).
阳性对照
WB: MCF7, Jurkat, HeLa, MOLT-4, HT-29 and Hep G2 cell lysate. IHC: Human stomach tissue.
MGMT Antibody (Ab115239) - Western Blot All lanes: MGMT Antibody (Ab115239) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa Observed band size: 24 kDa Exposure time: 2.6 s
MGMT Antibody (Ab115239) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10 µg/mL MGMT Antibody (Ab115239) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.