ExactAb™,已验证,重组,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human mH2A1 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/1000-1/5000
IF/ICC: 1/200
Protein Function:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
Recombinant mH2A1 Antibody (Ab115261) - Western Blot
All lanes: Recombinant mH2A1 Antibody (Ab115261) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/30000 dilution
Predicted band size: 39 kDa
Observed band size: 41 kDa
Exposure time: 45.3 s
Recombinant mH2A1 Antibody (Ab115261) - IF/ICC
IF analysis of Recombinant mH2A1 Antibody (Ab115261) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant mH2A1 Antibody (Ab115261) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/1000 - 1/5000
1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012186 |
分析证书 | Ab115261 |
| ZJ24F1012187 |
分析证书 | Ab115261 |
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