ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human MKRN2 Antibody, Monoclonal (F56S631), could be used for WB, IHC, IF, ICC, Flow and so on.
MKRN2 Mouse mAb (Ab115527) - Western Blot
All lanes: MKRN2 Mouse mAb (Ab115527) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 60.0 s
MKRN2 Mouse mAb (Ab115527) - IHC
Immunohistochemical analysis of Makorin-2 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MKRN2 Mouse mAb (Ab115527) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
MKRN2 Mouse mAb (Ab115527) - IF/ICC
Immunofluorescent analysis of Makorin-2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with MKRN2 Mouse mAb (Ab115527) in 3% BSA-PBS and incubated overnight at 4℃ in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MKRN2 Mouse mAb (Ab115527) - Flow
Flow cytometric analysis of K562 cells using MKRN2 Mouse mAb (Ab115527). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37℃ for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37℃ for 40 min. Isotype control antibody (blue line) was used under the same condition.
1/50-1/200
1/10 - 1/50
1/10-1/50
1/500-1/2000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012188 |
分析证书 | Ab115527 |
| ZJ24F1012189 |
分析证书 | Ab115527 |
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