MVK Antibody (Ab116570) - Western Blot All lanes: MVK Antibody (Ab116570) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 38.6 s
MVK Antibody (Ab116570) - Western Blot All lanes: MVK Antibody (Ab116570) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 38.6 s
MVK Antibody (Ab116570) - IF/ICC Immunofluorescent analysis of MVK staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with MVK Antibody (Ab116570) in 3% BSA-PBS and incubated overnight at 4 °C in a humidity chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
MVK Antibody (Ab116570) - IHC Immunohistochemical analysis of MVK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MVK Antibody (Ab116570) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.