Protein Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
规格
产品名称
Recombinant Nck Antibody
别名
NCK衔接蛋白1抗体 | 重组Nck抗体
英文别名
cytoplasmic protein NCK1 | MGC12668 | NCK adaptor protein 1melanoma NCK protein | NCK1 | nck-1 | NCKalpha | NCKNCK tyrosine kinase | non-catalytic region of tyrosine kinase | SH2/SH3 adaptor protein NCK-alpha | Cytoplasmic protein NCK1 antibody | MGC12668
规格或纯度
ExactAb™, Validated, 重组, 0.2 mg/mL
宿主种属
兔(Rabbit)
特异性
NCK1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Nck (AA 248-266)
阳性对照
WB: K562, HeLa, HEK293, A431, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Recombinant Nck Antibody (Ab117238) - Western Blot All lanes: Recombinant Nck Antibody (Ab117238) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 46 kDa Exposure time: 1.1 s
Recombinant Nck Antibody (Ab117238) - IF/ICC Immunocytochemistry analysis of NCK1 (green) in HeLa using Recombinant Nck Antibody (Ab117238), and cells were counterstained with DAPI (blue).
Recombinant Nck Antibody (Ab117238) - IHC Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant Nck Antibody (Ab117238). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.